Genes coding for integration host factor are conserved in gram-negative bacteria.

نویسندگان

  • H Haluzi
  • D Goitein
  • S Koby
  • I Mendelson
  • D Teff
  • G Mengeritsky
  • H Giladi
  • A B Oppenheim
چکیده

A genetic system for the selection of clones coding for integration host factor and HU homologs is described. We demonstrate that the himA and hip genes of Serratia marcescens and Aeromonas proteolytica can substitute for the Escherichia coli genes in a variety of biological assays. We find that the sequence and genetic organization of the himA and hip genes of S. marcescens are highly conserved.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

DETECTION OF BACTERIA BY AMPLIFYING THE 16S rRNA GENE WITH UNIVERSAL PRIMERS AND RFLP

Background: There is a conserved portion in the 16S rRNA gene of bacteria which can be amplified by the universal PCR method. This fragment is 996 bp in length. In this method, only one set of universal primers is used for the amplification of the conserved region of the 16S rRNA gene, in common bacterial pathogens. Therefore, using the universal PCR method, these bacteria are detectable on...

متن کامل

Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.

We describe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

متن کامل

Papaya Dieback in Malaysia: A StepTowards A New Insight of Disease Resistance

A recently published article describing the draft genome of Erwiniamallotivora BT-Mardi (1), the causal pathogen of papaya dieback infection in Peninsular Malaysia, hassignificant potential to overcome and reduce the effect of this vulnerable crop (2). The authors found that the draft genome sequenceis approximately 4824 kbp and the G+C content of the genomewas 52-54%, which is very similarto t...

متن کامل

ihfA gene of the bacterium Myxococcus xanthus and its role in activation of carotenoid genes by blue light.

Myxococcus xanthus responds to blue light by producing carotenoids. Several regulatory genes are known that participate in the light action mechanism, which leads to the transcriptional activation of the carotenoid genes. We had already reported the isolation of a carotenoid-less, Tn5-induced strain (MR508), whose mutant site was unlinked to the indicated regulatory genes. Here, we show that Om...

متن کامل

GENERATION OF A GENE CONSTRUCT TO SIPA GENE DELETION OF SALMONELLA TYPHIMURIUM

Background & Aims: Salmonella Typhimurium is a negative-gram, non-spore, free capsule, moving bacteria with Trish Perry flagella. Salmonella is the most common cause of food poisoning. The ability to enter and survive in host cells is the condition for pathogenic Salmonella species. Proteins of invasive Salmonella are transferred to the host cells by bacteria. This study was performed for gener...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 173 19  شماره 

صفحات  -

تاریخ انتشار 1991